Fluorescent Microscope Series - Almicro
Almicro Fluorescent Microscope Series - Specialized professional instruments engineered for advanced biological imaging, immuno fluorescence research, and venereal disease diagnosis. Available in two variants: AFM-100 (Reflected Fluorescence System) with 100W HBO Ultra-voltage Mercury Lamp and AFM-50 (Transmitted Fluorescence System) with 10W LED illumination. Both models feature trinocular/binocular heads with 30° incline, plan-achromatic objectives (4x-100x oil immersion), advanced filtering systems (Blue/Green/UV excitation), professional Abbe condensers, mechanical stages, and comprehensive fluorescence detection capabilities. Perfect for immunology laboratories, cell biology research, pathology departments, and medical diagnostic facilities. Trusted quality since 1978.
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Almicro Fluorescent Microscope Series - Advanced Fluorescence Imaging Solutions
The Almicro Fluorescent Microscope Series represents a specialized advancement in optical microscopy, engineered specifically for institutions engaged in cutting-edge biological research and diagnostics. These professional instruments combine sophisticated fluorescence detection systems with precision optical engineering to enable visualization of fluorescently-labeled molecules, cells, and tissue structures. With multiple fluorescence filter systems and advanced illumination technology, the AFM Series unlocks new dimensions in biological discovery.
Fluorescence Microscopy Overview
Fluorescence microscopy is a specialized optical technique that uses fluorescent labels to illuminate specimens. A fluorescence microscope detects and visualizes fluorescent signals, fluorescently-labeled molecules, and cells with fluorescently-labeled structures. This powerful technique enables:
- Detection of specific cellular structures and molecules via fluorescent markers
- Multi-channel imaging for simultaneous observation of multiple targets
- Enhanced contrast and specificity in biological specimens
- Immunofluorescence analysis of tissue antigens and disease markers
- Cell biology research at molecular resolution
- Pathological diagnosis of infectious and non-infectious diseases
Almicro's AFM Series is particularly suited for:
- Immunology and immunofluorescence research
- Cell and molecular biology laboratories
- Pathology departments conducting advanced diagnostics
- Venereal disease screening and diagnosis
- Bacterial and viral identification
- Clinical research institutions
- Medical colleges conducting advanced microscopy training
ALAMICRO FLUORESCENT MICROSCOPE SERIES OVERVIEW
AFM-100 - Reflected Fluorescence Microscope (Advanced Research)
Description:
The AFM-100 is a premium reflected fluorescence microscope using a powerful 100W HBO ultra-voltage spherical mercury lamp. It provides exceptional excitation efficiency for advanced fluorescence imaging, ideal for institutions requiring high sensitivity for fixed specimens and fluorescently-labeled tissue sections.
Optical & Viewing Systems:
• Compensation-free trinocular head inclined at 30°, interpupillary distance adjustable between 48-75mm.
• High point extra wide-field eyepieces (EW10x/20mm) and a trinocular port for camera documentation.
• Long working distance infinite plan achromatic objectives (4x/0.1 to 100x oil immersion 1.25 NA).
• Inverse quadruple nosepiece with click stops.
Condenser & Illumination:
• N.A. 1.25 rack and pinion adjustable condenser with a centering telescope for precise light path alignment.
• 100W HBO mercury lamp with adjustable brightness providing blue, green excitation filters, and ordinary bright field illumination for comparison.
• Magnification Range: 40x to 2500x (with oil immersion).
Mechanical Systems:
• Double layer mechanical stage (210mm x 40mm) with smooth X-Y movement and stainless steel clips.
• Coaxial coarse and fine focusing with ultra-precise 2μm minimum fine division.
Applications:
Immunofluorescence research, cell biology, pathology diagnostics, venereal disease diagnosis, bacterial and viral identification, teaching advanced fluorescence techniques.
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AFM-50 - Transmitted Fluorescence Microscope (Professional Research)
Description:
The AFM-50 offers professional fluorescence microscopy with transmitted light using 10W LED illumination. It features plan-achromatic objectives with antifungal and antireflection coatings and supports dual imaging modes—brightfield and fluorescence.
Optical & Viewing Systems:
• Sidetoph trinocular or binocular head inclined at 30°, 360° rotatable with interpupillary adjustment (48-75mm).
• Wide field 10x eyepieces (25mm FOV) with antifungal and anti-reflection coatings.
• Quadruple nosepiece with standard plan achromatic objectives (4x to 100x oil immersion), plus optional 20x, 40x, 60x, 80x.
Condenser & Illumination:
• Swing-out condenser N.A. 0.90 with iris diaphragm, frosted white filter, antifungal coating.
• 10W LED transmitted illumination with variable brightness, LED lifespan up to 60,000 hours. Simultaneous brightfield and fluorescence imaging.
• Magnification Range: Standard 40x–1000x, up to 4000x with optional objectives.
Mechanical Stage:
• Double layer ceramic hard-coated mechanical stage (210mm x 140mm) with X-Y travel (80mm x 70mm).
• Two-step coaxial coarse and fine focusing on both sides.
Applications:
Live cell imaging, immunofluorescence studies, pathology, venereal disease screening, fluorescent bacterial identification, research institutions, diagnostic and teaching centers.
Key Comparison: AFM-100 vs AFM-50
Feature | AFM-100 (Reflected Fluorescence) | AFM-50 (Transmitted Fluorescence) |
Fluorescence Type | Reflected Fluorescence | Transmitted Fluorescence |
Primary Illumination | 100W HBO Mercury Lamp | 10W LED |
Excitation Wavelengths | Multi-channel (Blue 420-485nm, Green 480-550nm, UV 330-400nm) | Single Immuno Fluorescence Filter |
Magnification Range | 40x to 2500x | 40x to 1000x standard, 4000x optional |
Viewing Head | Trinocular, 30° inclined | Trinocular/Binocular, 30° inclined, 360° rotatable |
Eyepieces | EW10x/20mm High Point Extra Wide Field | Wide Field 10x/25mm with antifungal & anti-reflection coating |
Stage Travel | 75mm x 50mm (smaller stage movement) | 80mm x 70mm (larger stage movement) |
Condenser | N.A. 1.25 Rack & Pinion with centering telescope | N.A. 0.90 Swing-out Abbe with iris diaphragm |
Imaging Modes | Fluorescence + Reflected Light | Brightfield + Fluorescence (simultaneous) |
LED Life | Not applicable (Mercury Lamp) | ~60,000 hours |
Power Supply | AV 220V | 110-240V AC universal (battery backup optional) |
Battery Backup | No | Optional |
Application Focus | Advanced research and immunofluorescence | Diagnostic centers and research flexibility |
Cost Category | Premium | Professional Entry |
Fluorescence Filter Systems
AFM-100 Multi-Channel Filters:
• System B (Blue 420-485nm) for FITC, GFP, Alexa Fluor 488.
• System G (Green 480-550nm) for Rhodamine, TRITC, Texas Red.
• System UV (330-400nm) for DAPI, Hoechst nuclear stains.
AFM-50 Single Channel Filter:
• Optimized for routine immunofluorescence diagnostics, LED-based for stability and long life.
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Maintenance & Care
• Careful cleaning of fluorescence filter surfaces with lens paper.
• Avoid touching mercury lamps with bare hands; careful handling essential.
• LED illumination requires minimal maintenance, with long service life.
• Remove oil immediately from objectives after use; clean optics with approved solutions.
• Maintain laboratory temperature 18-25°C for fluorescence stability.
• Annual professional calibration recommended along with optical alignment and anti-fungal inspection.
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Included with Both Models
• Microscope body with configured optical system.
• Specified eyepieces and plan achromatic objectives.
• Revolving nosepiece, mechanical stage with clips.
• Professional condensers and fluorescence filter sets.
• Illumination system (mercury lamp or LED) with power supply.
• High-grade immersion oil for 100x objectives.
• Protection dust cover, user manual, warranty, and professional packaging.