Microscopy chemicals

The following are the specifications for Azur II for microscopy:

Azure II dye comes as a blend of Azure B (sometimes referred to as Azure I) and Methylene Blue, mixed in equal parts. It's commonly used in blood staining, especially when paired with Eosin Y. Typically, nuclei take on a purple hue due to the way Eosin Y interacts with the Azure B-DNA combo. The intensity of this stain largely hinges on the quantity of Azure II used and the balance between Azure B and Eosin Y. However, keep in mind that the staining outcome can also be influenced by other elements, such as the pH of the solution, the type of buffer used, the fixation method, and how long the staining process lasts.

• CAS number: 53092-85-6
• Molecular formula: C16H18N3ClO6
• Molecular weight: 389.86 g/mol
• Appearance: Dark green to blackish powder
• Solubility: Soluble in water, ethanol, and methanol
• pH: 10.0-11.0
• Purity: 99.8%

Azur II is a safe and effective dye for microscopy. It is not known to be carcinogenic or mutagenic, and it has a low toxicity. Azur II should be stored in a cool, dark place and protected from light.

Here are some of the benefits of using Azur II for microscopy:

• It is a basic dye, which means that it stains negatively charged components of cells.
• It is a water-soluble dye, which makes it easy to prepare solutions.
• It is a relatively inexpensive dye.
• It is a safe and effective dye.