Azur II for Microscopy 10 gm 53092-85-6
The following are the specifications for Azur II for microscopy:
Azure II dye comes as a blend of Azure B (sometimes referred to as Azure I) and Methylene Blue, mixed in equal parts. It's commonly used in blood staining, especially when paired with Eosin Y. Typically, nuclei take on a purple hue due to the way Eosin Y interacts with the Azure B-DNA combo. The intensity of this stain largely hinges on the quantity of Azure II used and the balance between Azure B and Eosin Y. However, keep in mind that the staining outcome can also be influenced by other elements, such as the pH of the solution, the type of buffer used, the fixation method, and how long the staining process lasts.
- CAS number: 53092-85-6
- Molecular formula: C16H18N3ClO6
- Molecular weight: 389.86 g/mol
- Appearance: Dark green to blackish powder
- Solubility: Soluble in water, ethanol, and methanol
- pH: 10.0-11.0
- Purity: 99.8%
Azur II is a safe and effective dye for microscopy. It is not known to be carcinogenic or mutagenic, and it has a low toxicity. Azur II should be stored in a cool, dark place and protected from light.
Here are some of the benefits of using Azur II for microscopy:
- It is a basic dye, which means that it stains negatively charged components of cells.
- It is a water-soluble dye, which makes it easy to prepare solutions.
- It is a relatively inexpensive dye.
- It is a safe and effective dye.
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LABITEMS Azure II is a basic dye that is used in microscopy to stain cells and tissues. It is a mixture of azure I and methylene blue, and it is available as a dry powder or a liquid solution. Azure II is used to stain the nuclei of cells, and it can also be used to stain the cytoplasm and the cell membranes.
To use azure II for microscopy, you will need to prepare a staining solution. The staining solution is made by dissolving the azure II powder in water. The concentration of the staining solution will depend on the type of cells or tissues that you are staining. For general staining, a concentration of 0.1% azure II is usually sufficient.
Once the staining solution is prepared, you can add it to the cells or tissues that you want to stain. The cells or tissues should be incubated in the staining solution for a period of time, which will depend on the type of cells or tissues that you are staining. For general staining, an incubation time of 10-15 minutes is usually sufficient.
After the incubation period, the cells or tissues should be washed with water to remove the excess staining solution. The cells or tissues can then be mounted on a slide and viewed under a microscope.
Azure II is a versatile dye that can be used to stain a variety of cells and tissues. It is a relatively inexpensive dye, and it is easy to use. Azure II is a good choice for general staining of cells and tissues.
Here are some additional information about Azure II:
- Azure II is a basic dye, which means that it has a positive charge.
- Azure II is water soluble.
- Azure II is a pH indicator, and it changes color in the range of pH 10-11.
- Azure II is a relatively safe dye, but it can be irritating to the skin and eyes.
When working with Azure II, it is important to wear gloves and safety glasses. It is also important to work in a well-ventilated area.