Immersion oil is a specialized oil used in microscopy to improve the resolution and clarity of high-magnification images. It has a refractive index close to that of glass, which helps reduce light scattering and refraction, thereby enhancing the numerical aperture of the microscope objective. By filling the space between the microscope slide and the objective lens, immersion oil minimizes the loss of light rays and increases the amount of light collected, resulting in sharper and clearer images, especially at high magnifications. This technique, known as immersion microscopy, is essential for visualizing fine details and structures, such as individual cells, subcellular organelles, and microorganisms, with optimal clarity and resolution.
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