A small number of mosquitoes—typically five—are confined in a plastic cone sealed against the treated surface, exposing them to contact for a fixed period (3 minutes for nets, 30 minutes for walls). Knockdown is recorded at 60 minutes and mortality at 24 hours.

Mosquito larval surveys aim to obtain accurate information on mosquito occurrence, distribution, abundance, and ecology during their aquatic stages. The primary objectives are to identify active breeding habitats and determine mosquito species present therein

Mosquito rearing insectaries require precise environmental control, specialized cages, larval trays, feeding systems, and handling tools to ensure healthy colony growth.