Solvent: Chloroform is a widely used solvent for organic compounds, especially in extractions and purifications. It is commonly employed in nucleic acid extraction due to its ability to dissolve lipids and proteins, facilitating the separation of nucleic acids.

NMR Spectroscopy: Chloroform finds application as a solvent in Nuclear Magnetic Resonance (NMR) spectroscopy.

Reagent: Chloroform can be utilized as a reagent in specific chemical reactions.

Precautions while Handling Chloroform:

• Ventilation: Always use chloroform in a well-ventilated area or under a fume hood to prevent inhalation of fumes.
• Personal Protective Equipment (PPE): Wear suitable gloves, safety goggles, and a lab coat when handling chloroform to avoid skin contact and prevent splashes.
• Avoid Light and Heat: Store chloroform in a dark, cool place, away from direct sunlight and heat sources. Exposure to light and air can lead to the production of phosgene, a highly toxic compound.
• Ingestion: Never consume or taste chloroform. If ingested, seek immediate medical attention.
• Avoid Inhalation: Do not intentionally inhale chloroform vapors. Ensure you are working in a space with adequate air circulation.
• Waste Disposal: Dispose of chloroform waste in designated containers. Do not pour it down the drain or mix it with regular trash.
• Container Sealing: Always ensure that chloroform containers are tightly sealed to prevent evaporation and reduce exposure to air.
• Fire Hazard: Although chloroform is not highly flammable, it can form explosive mixtures with air. Keep it away from open flames, sparks, and ignition sources.
• Spill Handling: In case of a spill, increase ventilation in the area, wear appropriate PPE, and use inert absorbents to clean up the spill. Dispose of the absorbent material as hazardous waste.

• First Aid: Familiarize yourself with the first aid measures in case of chloroform exposure. Immediate action may be crucial in mitigating adverse health effects.