2?Mercaptoethanol AR/ Molecular Biology 100 ml CAS #1600?27?7
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₹1,357.50
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2?Mercaptoethanol AR/ Molecular Biology 100 ml CAS #1600?27?7
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2-Mercaptoethanol (also known as beta-mercaptoethanol or BME) is a potent reducing agent used in cell culture media to prevent toxic levels of oxygen radicals. 2-Mercaptoethanol is not stable in solution, so most protocols require daily supplementation. 2-Mercaptoethanol is used in some RNA isolation procedures to eliminate ribonuclease released during cell lysis. Numerous disulfide bonds make ribonucleases very stable enzymes, so 2-mercaptoethanol is used to reduce these disulfide bonds and irreversibly denature the proteins. This prevents them from digesting the RNA during its extraction procedure. 2-Mercaptoethanol (also ?-mercaptoethanol, BME, 2BME, 2-ME or ?-met) is the chemical compound with the formula HOCH2CH2SH. ME or ?ME, as it is commonly abbreviated, is used to reduce disulfide bonds and can act as a biological antioxidant by scavenging hydroxyl radicals (amongst others). It is widely used because the hydroxyl group confers solubility in water and lowers the volatility. Due to its diminished vapor pressure, its odor, while unpleasant, is less objectionable than related thiols.