Azur I for Microscopy 5 gm CAS #1562?90?9
Azure I is one of the thiazine dyes, like Azure B, and can be used in various staining procedures for microscopy, especially when combined with other dyes.
For instance:
Methylene blue-Azure II-Azure I (MAA) stains: This is used in cytological staining, especially for bone marrow and other cytological smears.
Romanowsky-type stains: Azure I can be a component in some of these formulations, which are utilized for blood smears and certain parasites. The combination of eosin with Azure dyes leads to the characteristic staining of cellular elements, where nuclei appear purple-blue, cytoplasm varies from pink to deep blue, and specific granules in certain white blood cells (like eosinophils) stain bright red.
Metachromasia: Azure I exhibits metachromatic staining properties. This means it can change color when binding to certain tissue components. For example, substances like heparin in mast cell granules can cause a shift in the color of the dye when stained, making these structures identifiable under the microscope.
In microscopy, Azure I, when used either alone or in combination with other dyes, allows for the differentiation of various cellular structures and elements, facilitating both research and clinical diagnostics.